Brightfield & Epifluorescence Microscopy

This facility is equipped with three microscopes: the Zeiss Axioplan upright, the Nikon E800 upright, and the Nikon TE300 inverted. Both Nikon microscopes are connected to cameras and equipped with Nikon NIS Element software for image acquisition. The Zeiss Axioplan upright is primarily used to confirm immunoreaction before image acquisition.

The facility has an automated stitching epifluorescence system, the Keyence, which is capable of imaging 4 channels (excitation at 405nm, 488nm, 561nm, 647nm) as well as brightfield and can scan three slides at a time. We also have three standard epifluorescent microscopes: the Zeiss Axioplan upright, the Nikon E800 upright, and the Nikon TE300 inverted. Both Nikon microscopes are connected to cameras and equipped with Nikon NIS Element software for image acquisition. The Zeiss Axioplan upright is primarily used to confirm immunoreaction before image acquisition.

  Keyence
 Zeiss Axioplan
Nikon E800
Nikon TE300
Configuration Inverted
Upright Upright Inverted
Objectives  
     2x
 X X
     4x/5x
 X X X
     10x
 X X X X
     20x
 X X X X
     40x
  X
     40x oil
 X X X
     60x oil
 X X X
Fluorophores
 
     DAPI
X
X X
     CFP
  X
     GFP/Alexa 488
 X X X X
     YFP
 X X
     Cy3/RFP/Texas Red
  X X
     Texas Red/mCherry
  X
     Cy5/Alexa 647
 X X