Agilent 2100 Bioanalyzer

The Agilent 2100 bioanalyzer is the industry standard for RNA sample QC and has replaced labor-intensive gel electrophoresis for this application. It is also rapidly replacing gel electrophoresis for DNA fragment analysis and SDS-PAGE analysis of protein samples.  Agilent 2100 Bioanalyzer

Miniaturization of analytical instrumentation has many advantages over conventional techniques.These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows. Launched in 1999, the Agilent 2100 bioanalyzer was the first commercially available instrument to use microfluidics technology for the analysis of biological samples.

The Agilent 2100 bioanalyzer offers a broad range of pre-validated analysis kits combined with an easy-to-use benchtop system

  • RNA quality check with RIN
    The industry standard for RNA analysis offering total
    RNA, mRNA and Small RNA’s data including RIN
    algorithm (RNA Integrity Number)
  • DNA size and quantity
    Smart, high resolution separation and quantification
    of DNA
  • SDS-PAGE replacement for Protein analysis
    The fast and reliable way to determine the quantity and
    purity of proteins from Coomassie down to silver stain
    sensitivity
Using Agilent 2100 Bioanalyzer

 

With brief training, the 2100 Bioanalyzer is a user operatable instrument. The user is responsible for all kits, chips, reagents and supplies required to use the instrument. Discounted kits and reagents are available via the core facility. Note: This Bioanalyzer does not have the flow cytometry option and is not compatible with reagents for this assay.

Fee

The Agilent 2100 Bioanalyzer is available for use free of charge. User is responsible for the cost of kits, reagents and supplies.

Key specifications for chips

(adapted from document supplied by Agilent)

 RNA analysis

RNA 6000 Nano

 RNA 6000 Pico
Small RNA
Total RNA mRNA
Total RNA 
mRNA
Quantitative range 25-500
ng/μL
25-500
ng/μL
- -
50-2000
pg/μL
Qualitative
range

5-5000
ng/μL

25-500
ng/μL
50-5000
pg/μL
250-5000
pg/μL
50-2000
pg/μL
Sizing range
 - -
-
-
6-150 nt
Anaylsis time
30 minutes
# of samples
12
12
11 11 11
Sample volume
1 μL
DNA analysis
DNA 1000
DNA 7500
DNA 12000
Sizing range
25 - 1000 bp
100 - 7500 bp
100 - 12000 bp
Sizing resolution

 25 - 100bp: 5bp
100 - 500bp: 5%
500 - 1000bp: 10%

100 - 1000bp: 5%
1000 - 7500bp: 15%
 100 - 1000bp: 5bp
1000 - 12000bp: 10%
Qualitative range
 0.1 - 50 ng/μL  0.1 - 50 ng/μL 0.1 - 50 ng/μL
Analysis time
 35 minutes
30 minutes
30 minutes
# of samples
12

Samle volume

 1 μL
 Protein analysis
Protein 80
Protein 230
High Sensitivity
Protein 250

Sizing range
5 - 80 kDa
14 - 230 kDa
10 -250 kDa
Sizing resolution
10%
Sensitivity
6-30 ng/μL
6-30 ng/μL
1 - 5 pg/μL
Quantitative range
60 - 2000 ng/μL
15 - 2000 ng/μL 0.3 - 3000 ng/μL
Qualitative range
6 - 4000 ng/μL
6 - 5000 ng/μL -
Analysis time
30 minutes
25 minutes
30 minutes
# of samples
10
Sample volume
4 μL
 

 

Please visit the manufacturer website for additional information.

Additional information

General information
Application guide

RNA analysis
RNA integrity
RNA integrity/ quantitation from LCM tissue samples

DNA analysis
(coming soon!)

Protein analysis
(coming soon!)