BTX ECM830 with HT-200 multi-well electroporation system

Electroporation is a powerful and economical mean to introduce macro-molecules, such as DNA, RNA, protein, etc, into living cells such as bacteria, yeast and mammalian cells. The square wave electroporation, as compared to exponential decay wave, has increased efficiencies and viability of mammalian cells.

BTX ECM830

The ECM 830 generator utilizes the new BTX Power Platform Technology and an all-new digital user interface. The versatility of the ECM 830 applications includes mammalian and plant cell transfections, embryo manipulation, nuclear transfer, and bacterial and yeast transformations. The ECM 830 possesses key features including a wide range of voltages from 5 to 3000 volts, finer voltage discrimination, pulse durations from 10 μsec to 10 sec, user control of pulse intervals, Arc Quenching™,digital display of actual voltage and pulse length delivered.
When the ECM 830 generator is paired with a HT-200 plate handles, it transformed into a high-through put electroporation system that allows the use of the multi-well electroporation system. HT 200 plate 
When the ECM 830 generator is paired with a HT-200 plate handles, it transformed into a high-through put electroporation system that allows the use of the multi-well electroporation system. This system allows users to boost their productivity by optimizing conditions and increasing yields and viability. Electroporate a 25 or 96 well plate in less than 1 minute! This system allows the users to quickly and efficiently optimize the electrical and biological parameters associated with any electroporation experiment. Now entire libraries can be screened rapidly and efficiently. The key to the system is the disposable multi-well plates. The HT-P25 is a 5 x 5, 25 well plate and the HT-96 is a standard 8 x 12, 96 well plate. These plates allow the user to prepare an entire set of experiments and run them. This approach minimizes required sample and waste materials.

Using the BTX ECM 830

The ECM 830 generator is a very user friendly instrument and requires minimal training before use. Please contact Albert Tai to schedule a meeting if you're using the instrument for the first time. 

Rate

Currently free of charge. The user is responsible for the cost of consumables, such as the electroporation cuvettes and plates.

Key specifications

ECM 830 square wave generator

Voltage range: 5 – 500 V LV Mode/ 1 V resolution
  30 – 3000 V HV Mode/ 5 V resolution
   
Pulse length range: 10 µs – 999 µs LV Mode/ 1 µs resolution
  1 msec – 999 msec LV Mode/ 1 msec resolution
  1 sec – 10 sec LV Mode/ 0.1 sec resolution
  10 µs – 600 µs HV Mode/ 1 µs resolution
   
Pulse interval: 100 msec - 10 sec
   
Multiple pulsing:  1 – 9
   

HT-200 High Throughput Electroporation Plate Handler

96 Well Plate Handler with Auto-Sense Track Switching
Voltage range:
 0 to 3000V DC (Pulse)
Pulse length range
 10 µsec to 10sec
Pulse Number range
 1 to 99
 

 

Click here for full specifications for the ECM830 and HT-200.

Please visit the manufacturer website for additional information.

Example protocols from BTX websites

Primary mammalian cells
Human peripheral blood lymphocytes (PBL)
Human dentritic cell
Human skin fibroblast
Human Umbilical vein endothelial cell (HUVEC)
Human T lymphocyte
Mouse thymocytes
Rat neonate neuronal cell
Chicken primary embryo fibroblast

Common mammalian cell lines
293T - Human embryonic kidney fibroblast
NIH-3T3 - mouse fibroblast
Jurkat - human T cell
CHO - Chinese hamster ovary
HepG2 - Human hepatocyte
Hela - Human cervical epithelial

Bacteria
E. coli XL1-Blue or DH5α

SCIID user contributed protocols
(Coming soon!)
• 293T - Human embryonic kidney fibroblast
• A20 - Mouse B cell lymphoma
• Jurkat - human T cell lymphoma

Other useful documents
General optimization protocol
Theoretical Conversion between square wave and exponential decay wave parameters
ECM 830 user manual
HT-200 user manual

For additional protocols, please visit manufacturer website