Biacore T100 - Surface plasmon resonance-based label free interaction study

Surface Plasmon Resonance, also widely known as SPR or Biacore, is standard technology for interactional study of biomolecules. This technology has the advantage of being able to work with biomolecules in their native forms, without the need of introduction of label or tag. The SPR-based technology continuously monitors the changes of mass deposition on the detector surface, and allows sensitive and reliable characterization of biomolecular interactions and provides information such as association/dissociation rate and affinity.  Biacore T100

Being the latest research model made by Biacore, the T100 is equipped with the most sensitive sensor that allows the study of interaction of even small compounds, such as small molecule inhibitors and peptides. The fully automated operation and independent temperature control for the detection chamber and sample chamber allows streamline and unattended operation for up to 4 days. This fully automated configuration allows user to perform assay containing large number of samples with minimal human intervention. The software package comes with T100 integrates the instrument control and data analysis. The software allows easy instrument operation and setting up unattended assays, the built-in operational protocols also advance operation, e.g. recovery of binding partner for downstream analysis and the analytical module allows automated data analysis.

Biacore T100 can be used primarily for kinetics study such as dissociation and association rate, and binding affinity. Yet, other interactional study such as epiptope mapping and thermodynamic has also been performed successfully on T100 platform. The SPR-based assay does not normally interfere by the color or complexity of the input samples, taken together with the high sensitivity and low sample volume requirement, makes the SPR platform a great tool for determination of target concentration in complex samples such as serum, urine and food samples.

Using the T100

The use of Biacore T100 currently requires a dedicated operator. Free (see Fee section below) "proof-of-concept" run is available. If you would like to use the Biacore T100 for your research, please contact Albert Tai for additional information.

Fee

Rate Table

  • The hourly rate covers some of the consumables such as tubes, caps, plates, sealing foils. The users are responsible for additional consumables including, but not limited to, sensor chips, coupling reagents, specialized running buffer and regeneration buffers.
  • For "proof-of-concept" run, users are required to provided the sensor chips and non-standard reagents.
  • Consulting (fee may apply) available upon request.

Key information & specifications

Analysis time per cycle
 Typically 2-15 minutes
Automation
 48 hours unattended operation
Sample type
 Low MW drug candidates to high MW proteins (also DNA, RNA, polysaccharides, lipids, cells and viruses), in various sample environment, e.g. DMSO-containing buffers, plasma and serum
Required sample volume
 Injection volume + 20-50µl (application dependent)
Injection volume
2-235 µl
Flow rate range
1 - 100 µl/min
Flow cell volume
0.06 µl
Flow cell height
40 µm
Sample/reagent capacity
1x96 or 384 well microplate + up to 33 reagent vials
Analysis temperature range
4-45 °C (maximum 20 degree below ambient temperature)
Sample storage temperature range
4-45 °C (maximum 15 degree below ambient temperature)
Sample refractive index range
1.33 - 1.39
Buffer selector
Automatic switching between 4 buffers
In-line reference substraction
Automatic
   
Typical working range
 
Kinetics:
 
Association rate constant (ka)
 103 - 107 M-1S-1 (and higher for macromolecular analytes)
Dissociation rate constant (kd)
 10-5 - 0.5 s-1
Sample concentration
 10-3 - 10-11 M
Molecular Weight detection
 >100 Da
Number of flow cells
4
Baseline noise
Typically <0.1 RU (RMS)
Baseline drift
Typically <0.3 RU/min
Recovery specification
1.5 µl analyte recovery volume
Please visit the manufacturer website for additional information.