Confocal Microscopy

SPE

Leica SPE

Four confocal microscopes are available, Leica SP8, Leica SP2, Leica SPE, and a Nikon A1R confocal.

The newest Leica SP8 microscope is attached to an inverted microscope, has an autofocusing feature and an automated stage capable of stitching or imaging multipoints, is equipped for live cell imaging, and has the following laser lines: 405nm, 457nm, 488nm, 514nm, 561nm, 594nm and 647nm. Our Leica SP2 and Leica SPE instruments are used in the upright configuration.

All Leica confocals are equipped with a spectral scanner system with a spectral range of detection from 400 to 850 nm. Excitation sources on the SP2 AOBS system include the argon laser generated 458nm, 475nm, 488nm, 514nm, a red diode 561nm, and a diode 633nm. The Leica SPE has all diode lasers including the 405nm, 488nm, 561nm, and 647nm.

The Nikon A1R confocal is attached to an inverted microscope, and has an automated stage capable of imaging multiple cells over time or stitching multiple image fields to obtain large images. It is also equipped with a stage top incubator and a Perfect Focus mechanism that supports live cell imaging and compensates for focus drift. There are two scanheads available, a point scanner (conventional) and a resonant scanner (30 frames/sec for 512x512 pixel field view). Simultaneous photoactivation/bleaching (using the 405nm laser) and imaging can be performed. Excitation lines include 405nm, 488/514nm, 561nm, and 647nm.

Whereas the Leica confocals are all capable of spectral detection where one has the ability to set an emission range, the emission filters on the Nikon collect the following ranges: 425-475nm (DAPI), 465-500nm (CFP), 500-550nm (GFP), 550-615nm (YFP), 570-620nm (RFP), 660-740nm (far red).


  Leica SP8  Leica SPE  Leica SP2 Nikon A1R Nikon TIRF
Excitation
         
     405nm  x x    x  
     440nm          x
     457nm  x    x  x  
     488nm  x  x  x  x  x
     514nm  x    x  x  x
     561nm
 x
 x  x  x  x
     594 nm
 x        
     639nm  x  x    x  x
Brightfield
 x  x  x  x  x
Live Imaging
 x    x  x  x
Water Immersion Lenses
   x  x    

Co-Directors
Philip Haydon, PhD
Michele Jacob, PhD

Core Manager
Alenka Lovy, PhD

Scientific Advisor
Kent Keyser, PhD
(University of Alabama, Birmingham)

Location
Stearns 207