TIRF microscopy


TIRF (total internal reflection flurorescence) microscopy produces very thin optical sections by creating an evanescent wave of excitation just 100nm above the coverslip without exciting molecules deeper within the specimen.  This results in optical sections much thinner than those obtained by the confocal microscope, but specimens must be within the evanescent excitation wave and must be mounted in aqueous media.  This inverted microscope is equipped with DIC and Perfect Focus, and does have a stage top incubator for live cell studies.  There is a choice of two cameras, an EM-CCD and an ORCA-ER CCD. 

The laser excitation lines available include 442nm, 488nm, 514nm, 561nm, and 639nm. 

Single channel imaging filter sets are available for 488 nm, 561 nm, and 639 nm excitable fluorochromes, and almost simultaneous dual channel imaging for 488 /561 nm is possible.  A quadruple filter cube (442/514/561/639nm) can be used for CFP/YFP/RFP/far red imaging. 

Rob Jackson, PhD
Michele Jacob, PhD

Core Manager
Alenka Lovy, PhD

Stearns 207